Analysis Reagents - List of Manufacturers, Suppliers, Companies and Products | IPROS GMS

[Next-Generation Sequencing Analysis Case] Analysis of Live Bacterial Microbial Community Structure

Introducing a case that demonstrates the method using EMA can analyze live microbial communities! *Case study collection available.

We will introduce community structure analysis using the selective membrane permeable dye EMA (in-house data). For environmental samples dominated by E. coli, we prepared samples that were heat-treated and sterilized, as well as untreated samples, and performed EMA treatment. Subsequently, DNA extraction was conducted for each sample, followed by 16S rRNA gene amplicon analysis. As a result, in the community structure analysis, E. coli, which had become dominant, was hardly detected in the heat-treated samples due to its death. Additionally, in the related links, we also introduce community structure analysis using reverse transcription cDNA from RNA (in-house data), so please take a look. 【Workflow】 ■ Prepare environmental samples (heat-treated, untreated) ■ EMA treatment ■ Light irradiation ■ DNA extraction ■ Creation of adapter library via EMA-PCR *For more details, please refer to the PDF document or feel free to contact us.